Antigen-Specific In Vivo Killing Assay

The in vivo killing assay allows the quantification of the antigen-specific killing capacity of Cytotoxic CD8 + T Lymphocytes (CTLs) in mice. CTLs are indeed known for the lysis of cells expressing foreign or modified antigen peptides on their MHC class I molecules. Here we describe the detailed protocol used for the in vivo specific lysis of cells expressing the H-2 K b immunodominant CD8 + T-cell epitope of the OVA protein: an 8 amino acid peptide corresponding to the 257-264 region of OVA (SIINFEKL).

Keywords: Antigen-specific lysis of target cells; Cytotoxic CD8+ T lymphocytes; Flow cytometry-based In vivo killing assay; Mouse model; Vaccine testing.

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